Standard Operating Procedures

I2I has collated a number of ‘best practice’ SOPs for the laboratory and semi-field testing of LLIN and IRS products from global testing sites. The SOPs have been reviewed by a panel of independent experts and are deliberately un-formatted for easy incorporation into site specific SOP files and adapted to location specific contexts.

5th April 2024

SOP: Methods for Performing Calculations and Dilutions

View

12th August 2022

SOP: Spraying with the potter tower

View

12th August 2022

SOP: IRS Block Preparation

View

11th August 2022

SOP: Cone bioassay of treated blocks

View

18th January 2023

SOP: Transportation of mosquitoes

View

18th January 2023

SOP: Scoring mosquitoes after hut IRS

View

18th January 2023

SOP: Removal of ant infestations from experimental huts

View

4th January 2023

SOP: Preparation of sugar-soaked cotton wool for feeding adult mosquitoes

View

12th August 2022

SOP: Preparation of experimental huts for evaluation of IRS

View

12th August 2022

SOP: Indoor residual spraying of experimental huts for evaluation of insecticides

View

12th August 2022

SOP: Cone bioassay on IRS treated surfaces in experimental huts

View

13th August 2024

SOP: Tunnel Test

View

13th August 2024

SOP: Net washing for laboratory LLIN trials

View

7th August 2024

SOP: Cone Bioassay

View

5th April 2024

SOP: Methods for Performing Calculations and Dilutions

View

17th August 2023

SOP: WHO wire ball bioassay

View

17th August 2023

SOP: Extraction of active ingredient (AI) from a treated filter paper

View

17th August 2023

SOP: HPLC quantification of surface and total active ingredient present on an ITN

View

17th August 2023

SOP: Net in tube bioassay – pyrethroid-chlorfenapyr nets

View

17th August 2023

SOP: Dissection and measurement of mosquito wings

View

18th July 2023

SOP: Intrinsic Toxicity

View

18th July 2023

SOP: Wind Tunnel Testing

View

10th May 2023

NIRI SOP: Assessment of Abrasion Resistance of Insecticide-Treated Nets (ITNs)

View

10th May 2023

NIRI SOP: Assessment of Hole Enlargement Resistance in Insecticide-Treated Nets (ITNs)

View

10th May 2023

NIRI SOP: Snag Strength Test for Insecticide-Treated Nets (ITNs)

View

14th July 2022

SOP: Methods for monitoring the durability of dual-AI insecticide-treated nets containing a pyrethroid plus pyriproxyfen (PPF)

View

14th July 2022

SOP: Methods for monitoring the durability of dual-AI insecticide-treated nets containing a pyrethroid plus piperonyl butoxide (PBO)

View

14th July 2022

SOP: Methods for monitoring the durability of dual-AI insecticide-treated nets containing a pyrethroid plus chlorfenapyr (CFP)

View

14th July 2022

SOP: Strain characterisation of resistant mosquitoes for monitoring bioefficacy in ITNs treated with two active ingredients (Dual-AI ITNs)

View

7th July 2022

SOP: Cutting and preserving net samples for chemical analysis

View

25th October 2023

SOP: Experimental hut preparation and running

View

17th August 2023

SOP: Ifakara Ambient Chamber Test (I-ACT)

View

17th August 2023

SOP: Dissection and measurement of mosquito wings

View

18th January 2023

SOP: Transportation of mosquitoes

View

18th January 2023

SOP: Removal of ant infestations from experimental huts

View

4th January 2023

SOP: Preparation of sugar-soaked cotton wool for feeding adult mosquitoes

View

4th January 2023

SOP: Mosquito collections from experimental huts

View

7th July 2022

SOP: Net washing for semifield trials

View

5th April 2024

SOP: Performing Larval Insecticide Bioassays and Larval Susceptibility Testing

View

18th July 2023

SOP: Insect Growth Regulator Larvicides

View

18th July 2023

SOP: Bacterial Larvicide Bioassay

View

18th July 2023

SOP: Field Evaluation of Microbial Mosquito Larvicide Efficacy

View

18th July 2023

SOP: Intrinsic Toxicity

View

18th July 2023

SOP: Semi-Field Trials: Open Field for Aerial Application

View

18th July 2023

SOP: Semi-Field Trials: Open Field for Ground Application

View

18th July 2023

SOP: Calibration for Aerial Application Equipment

View

18th July 2023

SOP: Calibration for Backpack and Truck-Mounted Sprayers

View

18th July 2023

SOP: Operational Field Trials: Ground and Aerial

View